Microbiological analysis of surfaces in butchers of the municipal market of Cuiabá/ Análisis microbiológico de superficies en carnicerías del mercado municipal de Cuiabá/ Análise microbiológica de superfícies em açougues do mercado municipal de Cuiabá

Autores

  • Evangreize Lara Centro Universitário UNIRONDON
  • Camila Laturner Felix Centro Universitário Cândido Rondon
  • Tiffany Dias Duarte Centro Universitário UNIRONDON
  • Viviane Karolina Vivi Centro Universitário UNIRONDON

Palavras-chave:

Commerce, Meat, Hygiene.

Resumo

Objective: to isolate enterobacteria, especially the thermotolerant, present on the surfaces of knives, trays and cutting boards used in the handling of meat in butchers. Method: cross-sectional study, conducted from April to October 2017 in nine establishments that sell meat products in Cuiabá – Mato Grosso, Brasil. Surface samples were collected from three different utensils: knife, board and tray. Results: Among utensils used on handling poultry meat, the knife got greater growth of enterobacteria (4,36x104 UFC/cm2). The cutting board intended for pork also reported higher 7,44x104 UFC/cm2. For beef, the tray and the board respectively, had scores of 2,5x105 UFC/cm2 and 2,41x105 UFC/cm2. The high growth of enterobacteria is justified by the grooves contained in these tools, poor maintenance and hygiene. It was also observed the presence of 27% of fecal coliforms and 73% of total coliforms. Conclusion: from the data obtained it is inferred that the hygienic and sanitary conditions are not in accordance with the RDC No. 216/2004.

Biografia do Autor

Evangreize Lara, Centro Universitário UNIRONDON

Laboratório Escola de Análises Clínicas - LEAC

Setor de microbiologia

Camila Laturner Felix, Centro Universitário Cândido Rondon

Laboratório Escola de Análises Clínicas - LEAC

Setor de microbiologia

Tiffany Dias Duarte, Centro Universitário UNIRONDON

Laboratório Escola de Análises Clínicas - LEAC

Setor de microbiologia

Viviane Karolina Vivi, Centro Universitário UNIRONDON

Laboratório Escola de Análises Clínicas - LEAC

Setor de microbiologia

Referências

Motarjemi Y, Kaferstein FK. Global estimation of foodborne diseases. World Health Stat Q. 1997; 50:5-11.

World Health Organization. World Health Organization global strategy for food safety: safer food for better health. Geneva, Switzerland: World Health Organization; 2002.

Tassew H, Abdissa A, Beyene G, Gebre-Selassie S. Microbial flora and food borne pathogens on minced meat and their susceptibility to antimicrobial agents. Ethiop J Health Sci. 2010; 20(3):137-43.

Garcia-Cruz CH, Hoffmann FL, Bueno SM. Monitoring of the hot dog sold in street in the central part of the city of Säo Paulo – SP. Hig Aliment. 2000; 14(75):48-51.

Tafesse F, Desse G, Bacha K, Alemayehu H. Microbiological quality and safety of street vended raw meat in Jijiga town of Somali Regional State, southeast Ethiopia. Afr J Microbiol Res. 2014; 8(48):3867-74.

Slorach SA. Integrated approaches to the management of food safety throughout the food chain. FAO/WHO Global Forum of Food Safety Regulators, Marrakesh, Morocco; 2002.

Lues JFR, Theron MM, Venter P, Rasephei MR. Microbial composition of bioarosols of a high throughout chicken slaughtering facility. Poult Sci. 2007; 86(1):142-9.

Brazil. Ministry of Health. Secretaria of Health Surveillance. Epidemiological surveillance of foodborne diseases in Brazil 2000 - 2013. Electronic Epidemiological Bulletin. Brasília: MS; 2013.

Brazil. Ministry of Health. Secretaria of Health Surveillance. National Notifiable Diseases system. Epidemiological data of period to 2000-2011. Brasília: MS; 2011.

Vanderzant C, Splittstoesser DF. Compendium of methods for the microbiological examination of foods. 3.ed. Washington: American Public Health Association; 1992.

Winn WC Jr, Allen S, Janda W, Koneman E, Procop G, Schreckenberger P, et al. Koneman’s color atlas and textbook of diagnostic microbiology. 6th ed. Philadelphia: Lippincott Williams and Wilkins; 2006.

Silva NJ, Junqueira VCA, Silveira NFA, Taniwaki MH, Santos RFS, Gomes RAR, Okazaki MM. Manual de métodos de análise microbiológica de alimentos. 3th ed. São Paulo: Varela; 2007.

Silva Jr EA. Manual de controle higiênico-sanitário em serviços de alimentação. 6th ed. São Paulo: Varela; 2008.

Brazil. Ministry of Health. National Health Surveillance Agency. Resolution - RDC n° 275 of 21 October 2002. Provides for the technical regulation of standard operating procedures applied to establishments producing/ industrializers food and the checklist of good manufacturing practices in establishments producers /industrializers food. Official Gazette of the Federative Republic of Brazil. Brasilia: MS; 2002.

Fasanmi GO, Olukole SG, Kehinde OO. Microbial studies of table scrapings from meat stalls in Ibadan Metropolis, Nigeria: Implications on meat hygiene. African J Bacteriol. 2010; 9(21):3158-62.

Haimanot T, Alemseged A, Beyene G. Microbial flora and food pathogens on mincemeat and their susceptibility to microbial agents. Ethiop J Health Sci. 2010; 20(3):137-43.

Hassan Ali N, Farooqui A, Khan A, Khan AY, Kazmi SU. Microbial contamination of raw meat and its environment in retail shops in Karachi, Pakistan. J Infect Dev Ctries. 2010; 4(6):382-8.

Gurmu EB, Gebretinsae H. Assessment of bacteriological quality of meat contact surfaces in selected butcher shops of Mekelle city, Ethiopia. J Environ Occup Sci. 2013; 2(2):61-6.

Subhan SA, Wahab A, Mujahid TY, Pirzada ZA, Naz SA, Khan I. Hygienic status of meat selling at road side shops in various areas of Karachi, Pakistan. Int J Adv Res. 2015; 3(1):1004-13.

Sudhakar GB, Paturkar AM, Waskar VS, Zende RJ. Bacteriological screening and environmental sources of contamination in abattoir and the meat shops in Mumbai, India. Asian J Food & agro-Industry. 2009; 2(03):280-90.

Kusumaningrum HD, Riboldi G, Hazelger WC, Beumer RR. Survival of foodborne pathogens on stainless steel surfaces and cross-contamination to foods. Int J Food Microbiol. 2003; 85(3):227-36.

Murphy KJ, Parker B, Dyer KA, Davis CR, Coates AM, Buckley JD, et al. A comparison of regular consumption of fresh lean pork, beef and chicken on body composition: a randomized cross-over trial. Nutrients. 2014; 6(2): 682–96.

Ak NO, Cliver DO, Kaspar CW. Decontamination of plastic and wooden cutting boards for kitchen use. J Food Protect. 1994; 57(1): 23–30.

Moore G, Blair IS, McDowell DA. Recovery and transfer of Salmonella Typhimurium from four different domestic food contact surfaces. J Food Prot. 2007; 70(10):2273-80.

Ravishankar S, Zhu L, Jaroni D. Assessing the cross contamination and transfer rates of Salmonella enterica from chicken to lettuce under different food-handling scenarios. Food Microbiology. 2010; 27(6):791–4.

Evans JA, Russel SL, James C, Corry JL. Microbiological contamination of food refrigeration equipment. J Food Eng. 2004; 62(3):225-32.

Costerton JW, Stewart PS, Greenberg EP. Bacterial Biofilms: A Common Cause of Persistent Infections. Science. 1999; 284(5418):1318-22.

Gorman R, Bloomfield S, Adley CC. A study of cross-contamination of food-borne pathogens in the domestic kitchen in the Republic of Ireland. Int J Food Microbiol. 2002; 76(1-2):143-50.

Brazil. Ministry of Agriculture and Supply. Ordinance No. 368 of 09.04.1997. Technical Regulation on sanitary conditions and good development practices for developers establishments/industrializers food. Official Gazette of the Federative Republic of Brazil, Brasília; 1997.

Corcoran M, Morris D, De Lappeb N, O'Connor J, Lalor P, Dockery P, et al. Commonly Used Disinfectants Fail To Eradicate Salmonella Enterica Biofilms from Food Contact Surface Materials. Appl Environ Microbiol. 2014; 80(4):1507–14.

Bessems E. The effect of practical conditions on the efficacy of disinfectants. Int Biodeterior Biodegrad. 1998; 41:177-83.

Lambrechts AA, Human IS, Doughari JH, Lues JFR. Bacterial contamination of the hands of food handlers as indicator of hand washing efficacy in some convenient food industries in South Africa. Pak J Med Sci. 2014; 30(4):755–8.

Zerabruk K, Retta N, Muleta D, Tefera AT. Assessment of Microbiological Safety and Quality of Minced Meat and Meat Contact Surfaces in Selected Butcher Shops of Addis Ababa, Ethiopia. J Food Qual. 2019; (53):1-9.

Brazil. Resolution RDC n° 216 of 15/09/2004. Technical Regulation of Good Manufacturing Practices for food services. Official Gazette of the Federative Republic of Brazil. Brasília: MS; 2004.

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Publicado

2019-12-01

Como Citar

Lara, E., Felix, C. L., Duarte, T. D., & Vivi, V. K. (2019). Microbiological analysis of surfaces in butchers of the municipal market of Cuiabá/ Análisis microbiológico de superficies en carnicerías del mercado municipal de Cuiabá/ Análise microbiológica de superfícies em açougues do mercado municipal de Cuiabá. Journal Health NPEPS, 4(2), 253–267. Recuperado de https://periodicos.unemat.br/index.php/jhnpeps/article/view/4047

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Artigo Original/ Original Article/ Artículo Originale

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